This new toolbox offers the complete lymphoma service for the diagnosis and monitoring of canine lymphoma.
The combination of PARR with the canine Lymphoma Blood Test (cLBT) is an exciting new development which provides a unique service comprising the most advanced diagnostic and prognostic testing with remission and recurrence monitoring. Our partner in this development is CTDS, part of VPG, who uniquely offer combined FNA cytology and PARR on the same slide. Additionally, since cLBT is proprietary to Avacta Animal Health, we are launching a genuinely unique, combined service in veterinary oncology taking lymphoma diagnosis and monitoring to the next level.
Lymphoma remains one of the most common neoplasms seen in dogs. Unfortunately, the long-term prognosis has always been considered poor in the majority of cases.
However, in the last few years, significant advances have been made in the diagnosis, therapy and monitoring resulting in improvements to the outcome for individual cases. Many of these advances allow lymphoma to be better diagnosed and treated in first opinion practices without the need for referral.
Advanced molecular tests such as PARR (PCR for Antigen Receptor Rearrangement), can confirm the presence of a clonal lymphoid population (a characteristic of neoplasia) and allow the genetic abnormalities associated with T and B cell lymphomas to be identified. This information is important for prognostic assessment and treatment planning since high grade B cell lymphomas show better responses to chemotherapy drugs and tend to exhibit longer survival times than their T cell equivalents (see Table 1).
Table 1. Reference: A.M. Frantz, A.L. Sarver, D. Ito, et al. Molecular Profiling Reveals Prognostically Significant Subtypes of Canine Lymphoma. Vet Pathol. 2013 Jul; 50(4): 693-703.
*1 dog classified as intermediate grade was censored from the analysis.
There are currently two methods for differentiating T cell and B cell lymphomas in lymph node aspirates, flow cytometry and PARR. The former looks at markers on the surface of live lymphocytes and requires specialist shipping of samples to the laboratory, whereas PARR can be performed using FNA smears dried onto microscope slides and combined with routine cytology. Consequently, PARR can provide clonality and immunophenotype information on samples that are conveniently and routinely prepared by vets in practice, which can negate the need for intrusive lymph node biopsies. Cytology and PARR are also available for your feline patients. PARR is particularly useful for some feline lymphomas where neoplastic cells closely resemble normal lymphocytes.
PARR, in conjunction with cytology, ensures a superior classification of lymphomas. This, together with clinical staging, enables vets in practice to give owners a more accurate diagnosis and prognosis for their pet, both in terms of life expectancy and the likelihood of achieve sustained remission.
WHEN TO USE:
When cytology results are diagnostic for, or suspicious of lymphoma, PARR can confirm the immunophenotype from the same slide. The cLBT can then be used to monitor for treatment response and sensitively detect the onset of disease recurrence.
If the slides are not diagnostic for lymphoma, additional recommendations for investigation will be included in the report. Up to 5 cytology slides will be examined for a single fee, with additional slides charged separately.
With the cLBT and PARR in combination, we now have powerful, new tools to give a much clearer picture of the likely outlook for an individual patient, so you can guide and support owners’ decisions in relation to therapy and management.
HOW TO SUBMIT A SAMPLE:
- Take aspirate – smear cytology aspirates onto labelled slides.
- Smears should be air dried, please do not stain or use a cover slip.
- Complete cytology/PARR request form, download the submission form here.
- Please submit slides in slide mailer and packed in a seperate biohazard bag. N.B these fit in an Avacta sub box.
- Send to Avacta Animal Health using the freepost envelope provided within the submission box.
Cytology report: within 48 hours depending on receipt of sample.
PARR: 2-6 working days depending on receipt of sample.
cLBT: within 24 hours depending on receipt of sample.
For more information or to request a submission form please contact us.